HA722521

Somatostatin Receptor 2 Recombinant Rabbit Monoclonal Antibody [JE03-37]

cat.: HA722521

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, IF-Tissue, IHC-Fr
Clonality:	Monoclonal
Clone number:	JE03-37

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 41 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within human Somatostatin Receptor 2 aa 320-369 / 369.
Positive control:	SH-SY5Y cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, mouse brain tissue, human brain tissue, rat brain tissue.
Subcellular location:	Cell membrane.
Recommended Dilutions: WB IHC-P IF-Tissue IHC-Fr	1:1,000 1:200 1:100 1:500
Uniprot #:	SwissProt: P30874 Human \| P30875 Mouse \| P30680 Rat
Alternative names:	Somatostatin receptor type 2 SRIF-1 SRIF1 SS-2-R SS2-R SS2R SSR2_HUMAN SST2 SSTR2

Images

	Fig1: Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722521, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
	Fig2: Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722521, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
	Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722521) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig4: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722521) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA722521) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Somatostatin Receptor 2 with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722521, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Fig7: Western blot analysis of Somatostatin Receptor 2 on different lysates with Rabbit anti-Somatostatin Receptor 2 antibody (HA722521) at 1/1,000 dilution.

Lane 1: SH-SY5Y cell lysate
Lane 2: SH-SY5Y cell lysate (no heat)
Lane 3: Mouse brain tissue lysate (no heat)
Lane 4: Rat brain tissue lysate (no heat)

Notice: no heat means the lysate is not boiled.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 41 kDa
Observed band size: 60-75 kDa

Exposure time: Lane 1-2: 2 minutes; Lane 3-4: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722521) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.