PKM1/2 Recombinant Rabbit Monoclonal Antibody [JE07-34]
cat.: HA722524
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JE07-34 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PKM aa 50-100. |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse skeletal muscle tissue lysate, Rat skeletal muscle tissue lysate. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IP |
1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P14618 human | P52480 Mouse | P11980 Rat |
| Alternative names: | CTHBP Cytosolic thyroid hormone-binding protein KPYM_HUMAN OIP-3 Opa-interacting protein 3 p58 pkm PKM1 PKM2 Pyruvate kinase 2/3 Pyruvate kinase muscle isozyme Pyruvate kinase PKM THBP1 Thyroid hormone-binding protein 1 Tumor M2-PK |
Images
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Fig1:
Western blot analysis of PKM1/2 on different lysates with Rabbit anti-PKM1/2 antibody (HA722524) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: MCF7 cell lysate (20 µg/Lane) Lane 4: NIH/3T3 cell lysate (20 µg/Lane) Lane 5: C6 cell lysate (20 µg/Lane) Lane 6: Mouse skeletal muscle tissue lysate (30 µg/Lane) Lane 7: Rat skeletal muscle tissue lysate (30 µg/Lane) Predicted band size: 58 kDa Observed band size: 58 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722524) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
PKM1/2 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722524 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722524 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA722524 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA722524 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 2 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.