CDCA3 Recombinant Rabbit Monoclonal Antibody [JE08-65]
cat.: HA722525
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE08-65 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CDCA3 aa 1-50 / 268. |
| Positive control: | Caco-2 cell lysate, A431 cell lysate, HDLM-2 cell lysate, A549 cell lysate, SiHa cell lysate. |
| Subcellular location: | Cytoplasm, cytosol. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q99618 Human |
| Alternative names: | C8 CDCA 3 Cell division cycle associated 3 Cell division cycle associated protein 3 Gene rich cluster C8 Gene rich cluster protein C8 GRCC 8 GRCC8 MGC2577 TOME 1 TOME1 Trigger of mitotic entry 1 Trigger of mitotic entry protein 1 |
Images
|
Fig1:
Western blot analysis of CDCA3 on different lysates with Rabbit anti-CDCA3 antibody (HA722525) at 1/1,000 dilution. Lane 1: Caco-2 cell lysate Lane 2: A431 cell lysate Lane 3: HDLM-2 cell lysate Lane 4: A549 cell lysate Lane 5: SiHa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722525) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.