IGF1 Recombinant Rabbit Monoclonal Antibody [JE30-01]
cat.: HA722527
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE30-01 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human IGF1 aa 91-140 / 195. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P05019 Human |
| Alternative names: | IBP1 IGF I IGF IA IGF IB IGF-I Igf1 IGF1_HUMAN IGF1A IGFI IGFIA Insulin like growth factor 1 (somatomedin C) Insulin like growth factor 1 Insulin like growth factor IA Insulin like growth factor IB Insulin-like growth factor I Mechano growth factor MGF OTTHUMP00000195080 OTTHUMP00000195081 OTTHUMP00000195082 OTTHUMP00000195083 OTTHUMP00000195084 Somatomedia C Somatomedin C Somatomedin-C |
Images
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Fig1:
Western blot analysis of IGF1 on different lysates with Rabbit anti-IGF1 antibody (HA722527) at 1/1,000 dilution. Lane 1: 293T-NT cell lysate Lane 2: 293T-OE-IGF1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 22 kDa Observed band size: 25 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722527) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.