PCSK9 Recombinant Rabbit Monoclonal Antibody [JE30-37]
cat.: HA722528
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JE30-37 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 74 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PCSK9 aa 550-590/692. |
| Positive control: | Huh7 cell lysate, BxPC-3 cell lysate, HeLa cell lysate, A431 cell lysate, human colon cancer tissue, human kidney tissue, human pancreas tissue. |
| Subcellular location: | Cytoplasm, Secreted, Endosome, Lysosome, Cell surface, Endoplasmic reticulum, Golgi apparatus. |
| Recommended Dilutions:
WB IHC-P IP |
1:1,000 1:200 1-2μg/sample |
| Uniprot #: | SwissProt: Q8NBP7 Human |
| Alternative names: | Convertase subtilisin/kexin type 9 preproprotein FH3 HCHOLA3 Hypercholesterolemia autosomal dominant 3 LDLCQ1 NARC 1 NARC-1 NARC1 Neural apoptosis regulated convertase 1 Neural apoptosis-regulated convertase 1 PC 9 PC9 PCSK 9 PCSK9 PCSK9_HUMAN Proprotein convertase 9 Proprotein convertase PC9 Proprotein convertase subtilisin/kexin type 9 PSEC0052 Subtilisin/kexin like protease PC9 Subtilisin/kexin-like protease PC9 |
Images
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Fig1:
Western blot analysis of PCSK9 on different lysates with Rabbit anti-PCSK9 antibody (HA722528) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (negative) Lane 2: Huh7 cell lysate Lane 3: BxPC-3 cell lysate Lane 4: HeLa cell lysate Lane 5: A431 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 74 kDa Observed band size: 60/74 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722528) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-PCSK9 antibody (HA722528) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722528) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PCSK9 antibody (HA722528) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722528) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-PCSK9 antibody (HA722528) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722528) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.