ALK-1 Recombinant Rabbit Monoclonal Antibody [JE30-65]
cat.: HA722529
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE30-65 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ALK-1 aa 454-503 / 503. |
| Positive control: | HeLa cell lysate, Human lung tissue lysate, Mouse lung tissue lysate, Rat lung tissue lysate, human lung tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P37023 Human | Q61288 Mouse | P80203 Rat |
| Alternative names: | Activin A receptor Activin A receptor type II like 1 Activin A receptor, type II like kinase 1 Activin receptor like kinase 1 Activin receptor-like kinase 1 ACVL1_HUMAN ACVRL1 ACVRLK1 ALK-1 ALK1 HHT HHT2 ORW2 Osler Rendu Weber syndrome 2 Serine/threonine protein kinase receptor R3 Serine/threonine-protein kinase receptor R3 SKR3 TGF B superfamily receptor type I TGF-B superfamily receptor type I TSR-I TSR1 |
Images
|
Fig1:
Western blot analysis of ALK-1 on different lysates with Rabbit anti-ALK-1 antibody (HA722529) at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: Human lung tissue lysate (40 µg/Lane) Lane 3: Mouse lung tissue lysate (40 µg/Lane) Lane 4: Rat lung tissue lysate (40 µg/Lane) Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722529) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-ALK-1 antibody (HA722529) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722529) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.