ITCH / AIP4 Recombinant Rabbit Monoclonal Antibody [JE31-09]
cat.: HA722530
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE31-09 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 103 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ITCH / AIP4 aa 1-50 / 903. |
| Positive control: | SiHa cell lysate, HeLa cell lysate, Ramos cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate. |
| Subcellular location: | Cell membrane, Cytoplasm, Nucleus, Early endosome membrane, Endosome membrane. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: Q96J02 Human | Q8C863 Mouse Entrez Gene: 311567 Rat |
| Alternative names: | ADMFD AIF4 AIP4 Atrophin 1 interacting protein 4 Atrophin-1-interacting protein 4 dJ468O1.1 dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)) dJ468O1.1 atrophin 1 interacting protein 4 AIP4 E3 ubiquitin protein ligase Itchy homolog E3 ubiquitin-protein ligase Itchy homolog EC 6.3.2 Itch ITCH_HUMAN Itchy E3 ubiquitin protein ligase Itchy E3 ubiquitin protein ligase homolog Itchy E3 ubiquitin protein ligase homolog mouse Itchy E3 ubiquitin protein ligase, mouse, homolog of Itchy homolog E3 ubiquitin protein ligase Itchy mouse homolog E3 ubiquitin protein ligase NAPP1 NFE2 associated polypeptide 1 NFE2-associated polypeptide 1 Ubiquitin protein ligase ITCH |
Images
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Fig1:
Western blot analysis of ITCH / AIP4 on different lysates with Rabbit anti-ITCH / AIP4 antibody (HA722530) at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: HeLa cell lysate Lane 3: Ramos cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: C6 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 103 kDa Observed band size: 103 kDa Exposure time: 45 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722530) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ITCH / AIP4 on different lysates with Rabbit anti-ITCH / AIP4 antibody (HA722530) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-ITCH / AIP4 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 102 kDa Observed band size: 102 kDa Exposure time: 90 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722530) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.