Follistatin Recombinant Rabbit Monoclonal Antibody [JE31-78]
cat.: HA722532
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE31-78 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein. |
| Positive control: | C6 cell lysate, Mouse ovary tissue lysate, Rat ovary tissue lysate, mouse ovary tissue, rat ovary tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P19883 Human | P47931 Mouse | P21674 Rat |
| Alternative names: | Activin binding protein Activin-binding protein Follistatin FS fst FST_HUMAN Human follistatin gene |
Images
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Fig1:
Western blot analysis of Follistatin on different lysates with Rabbit anti-Follistatin antibody (HA722532) at 1/1,000 dilution. Lane 1: C6 cell lysate (20 µg/Lane) Lane 2: Mouse ovary tissue lysate (30 µg/Lane) Lane 3: Rat ovary tissue lysate (30 µg/Lane) Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722532) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-Follistatin antibody (HA722532) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722532) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-Follistatin antibody (HA722532) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722532) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.