MAT2A Recombinant Rabbit Monoclonal Antibody [JE33-45]
cat.: HA722536
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JE33-45 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human MAT2A aa 71-120 / 395. |
| Positive control: | A549 cell lysate, NCI-H226 cell lysate, HeLa cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, Neuro-2a cell lysate, C6 cell lysate, human colon tissue, mouse colon tissue, rat colon tissue. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:500-1:2,000 1:100-1:500 |
| Uniprot #: | SwissProt: P31153 Human | Q3THS6 Mouse | P18298 Rat |
| Alternative names: | AdoMet synthase 2 AdoMet synthetase 2 AdoMet synthetase AMS 2 AMS2 MAT 2 MAT 2A MAT II MAT-II Mat2a MATA 2 MATA2 MATII Methionine adenosyltransferase 2 Methionine adenosyltransferase Methionine adenosyltransferase II alpha Methionine adenosyltransferase II METK2_HUMAN S adenosylmethionine synthetase gamma form S adenosylmethionine synthetase isoform type 2 S-adenosylmethionine synthase isoform type-2 SAMS 2 SAMS2 |
Images
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Fig1:
Western blot analysis of MAT2A on different lysates with Rabbit anti-MAT2A antibody (HA722536) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: NCI-H226 cell lysate Lane 3: HeLa cell lysate Lane 4: HepG2 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: Neuro-2a cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 44/46 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722536) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MAT2A antibody (HA722536) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722536) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-MAT2A antibody (HA722536) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722536) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-MAT2A antibody (HA722536) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722536) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.