Guanylyl Cyclase alpha 1 Recombinant Rabbit Monoclonal Antibody [JE34-21]
cat.: HA722537
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE34-21
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 77 kDa
Isotype: IgG
Immunogen: Recombinant protein within
Positive control: HEK-293 cell lysate, SH-SY5Y cell lysate.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q02108 Human
Alternative names: GC S alpha 1 GC SA3 GCS alpha 1 GCS alpha 3 GCS-alpha-1 GCS-alpha-3 GCYA3_HUMAN Guanylate cyclase 1 soluble alpha 3 Guanylate cyclase soluble subunit alpha 1 Guanylate cyclase soluble subunit alpha 3 Guanylate cyclase soluble subunit alpha-3 Guanylate cyclase, nitric oxide-sensitive, alpha-1 subunit Guc1a1 GUC1A3 GUCA3 GUCSA3 Gucy1a1 Gucy1a3 MYMY6 nitric oxide-sensitive Guanylate cyclase, alpha-1 subunit NOGC, alpha-1 subunit Soluble guanylate cyclase large subunit
Images
HA722537_1.jpg Fig1: Western blot analysis of Guanylyl Cyclase alpha 1 on different lysates with Rabbit anti-Guanylyl Cyclase alpha 1 antibody (HA722537) at 1/1,000 dilution.

Lane 1: HEK-293 cell lysate
Lane 2: SH-SY5Y cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 77 kDa
Observed band size: 75 kDa

Exposure time: 59 seconds; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722537) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.