Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE34-21 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 77 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within |
Positive control: | HEK-293 cell lysate, SH-SY5Y cell lysate. |
Subcellular location: | Cytoplasm. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: Q02108 Human |
Alternative names: | GC S alpha 1 GC SA3 GCS alpha 1 GCS alpha 3 GCS-alpha-1 GCS-alpha-3 GCYA3_HUMAN Guanylate cyclase 1 soluble alpha 3 Guanylate cyclase soluble subunit alpha 1 Guanylate cyclase soluble subunit alpha 3 Guanylate cyclase soluble subunit alpha-3 Guanylate cyclase, nitric oxide-sensitive, alpha-1 subunit Guc1a1 GUC1A3 GUCA3 GUCSA3 Gucy1a1 Gucy1a3 MYMY6 nitric oxide-sensitive Guanylate cyclase, alpha-1 subunit NOGC, alpha-1 subunit Soluble guanylate cyclase large subunit |
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Fig1:
Western blot analysis of Guanylyl Cyclase alpha 1 on different lysates with Rabbit anti-Guanylyl Cyclase alpha 1 antibody (HA722537) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: SH-SY5Y cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 77 kDa Observed band size: 75 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722537) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |