| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE34-21 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 77 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Guanylyl Cyclase alpha 1 aa 1-200 / 690. |
| Positive control: | HEK-293 cell lysate, SH-SY5Y cell lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q02108 Human |
| Alternative names: | GC S alpha 1 GC SA3 GCS alpha 1 GCS alpha 3 GCS-alpha-1 GCS-alpha-3 GCYA3_HUMAN Guanylate cyclase 1 soluble alpha 3 Guanylate cyclase soluble subunit alpha 1 Guanylate cyclase soluble subunit alpha 3 Guanylate cyclase soluble subunit alpha-3 Guanylate cyclase, nitric oxide-sensitive, alpha-1 subunit Guc1a1 GUC1A3 GUCA3 GUCSA3 Gucy1a1 Gucy1a3 MYMY6 nitric oxide-sensitive Guanylate cyclase, alpha-1 subunit NOGC, alpha-1 subunit Soluble guanylate cyclase large subunit |
|
Fig1:
Western blot analysis of Guanylyl Cyclase alpha 1 on different lysates with Rabbit anti-Guanylyl Cyclase alpha 1 antibody (HA722537) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: SH-SY5Y cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 77 kDa Observed band size: 75 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722537) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |