NPR-C Recombinant Rabbit Monoclonal Antibody [JE34-50]
cat.: HA722538
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE34-50 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide. |
| Positive control: | HeLa cell lysate, Human lung tissue lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, HeLa. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB FC |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P17342 Human | P70180 Mouse | P41740 Rat |
| Alternative names: | ANP C ANP-C ANPC ANPR-C ANPRC ANPRC_HUMAN Atrial natriuretic peptide C type receptor Atrial natriuretic peptide clearance receptor Atrial natriuretic peptide receptor 3 Atrial natriuretic peptide receptor type C Atrionatriuretic peptide receptor C C5orf23 FLJ14054 GUCY2B MGC22189 Natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C) Natriuretic peptide receptor C/guanylate cyclase C NPR 3 NPR C NPR-C Npr3 NPRC |
Images
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Fig1:
Western blot analysis of NPR-C on different lysates with Rabbit anti-NPR-C antibody (HA722538) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Human lung tissue lysate (40 µg/Lane) Lane 3: Mouse kidney tissue lysate (40 µg/Lane) Lane 4: Rat kidney tissue lysate (40 µg/Lane) Predicted band size: 60 kDa Observed band size: 60/70 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722538) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of HeLa cells labeling NPR-C. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722538, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.