Oxytocin Receptor Recombinant Rabbit Monoclonal Antibody [JE34-57]
cat.: HA722539
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE34-57
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 43 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Oxytocin Receptor aa 98-113 / 389.
Positive control: U-2 OS cell lysate, Mouse testis tissue lysate, Mouse skeletal muscle tissue lysate, Rat uterus tissue lysate, Rat skeletal muscle tissue lysate, Rat testis tissue lysate.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P30559 Human | P97926 Mouse | P70536 Rat
Alternative names: OT R OT-R OTR 1 OTR OTR1 OXT R Oxtr OXYR_HUMAN Oxytocin receptor
Images
HA722539_1.jpg Fig1: Western blot analysis of Oxytocin Receptor on different lysates with Rabbit anti-Oxytocin Receptor antibody (HA722539) at 1/1,000 dilution.

Lane 1: U-2 OS cell lysate
Lane 2: Mouse testis tissue lysate
Lane 3: Mouse skeletal muscle tissue lysate
Lane 4: Rat uterus tissue lysate
Lane 5: Rat skeletal muscle tissue lysate
Lane 6: Rat testis tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 43 kDa
Observed band size: 50 kDa

Exposure time: 1 minute 2 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722539) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.