| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE34-57 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Oxytocin Receptor aa 98-113 / 389. |
| Positive control: | U-2 OS cell lysate, Mouse testis tissue lysate, Mouse skeletal muscle tissue lysate, Rat uterus tissue lysate, Rat skeletal muscle tissue lysate, Rat testis tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P30559 Human | P97926 Mouse | P70536 Rat |
| Alternative names: | OT R OT-R OTR 1 OTR OTR1 OXT R Oxtr OXYR_HUMAN Oxytocin receptor |
|
Fig1:
Western blot analysis of Oxytocin Receptor on different lysates with Rabbit anti-Oxytocin Receptor antibody (HA722539) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: Mouse testis tissue lysate Lane 3: Mouse skeletal muscle tissue lysate Lane 4: Rat uterus tissue lysate Lane 5: Rat skeletal muscle tissue lysate Lane 6: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 50 kDa Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722539) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |