VPS24 Recombinant Rabbit Monoclonal Antibody [JE34-58]
cat.: HA722540
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE34-58 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human VPS24 aa 50-222 / 222. |
| Positive control: | HepG2 cell lysate, U-87 MG cell lysate, Human brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cytoplasm, cytosol, Membrane, Endosome, Late endosome membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q9Y3E7 Human | Q9CQ10 Mouse | Q8CGS4 Rat |
| Alternative names: | VPS 24 VPS24 CGI 149 CGI149 protein Charged multivesicular body protein 3 CHMP3 CHMP3_HUMAN Chromatin modifying protein 3 Chromatin-modifying protein 3 HGNC:29865 hVps24 NEDF Neuroendocrine differentiation factor Vacuolar protein sorting 24 (yeast) Vacuolar protein sorting 24 homolog (S. cerevisiae) Vacuolar protein sorting associated protein 24 Vacuolar protein sorting-associated protein 24 vps24 |
Images
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Fig1:
Western blot analysis of VPS24 on different lysates with Rabbit anti-VPS24 antibody (HA722540) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: U-87 MG cell lysate (20 µg/Lane) Lane 3: Human brain tissue lysate (40 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Lane 5: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722540) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.