CaMKI Recombinant Rabbit Monoclonal Antibody [JE36-77]
cat.: HA722544
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE36-77 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CaMKI aa 1-50 / 370. |
| Positive control: | HEK-293 cell lysate, SH-SY5Y cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Human brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, NIH/3T3, PC-12. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: Q14012 Human | Q91YS8 Mouse | Q63450 Rat |
| Alternative names: | Calcium / calmodulin dependent protein kinase 1 Calcium / calmodulin dependent protein kinase I Calcium / calmodulin dependent protein kinase type 1 Calcium/calmodulin dependent protein kinase 1 Calcium/calmodulin dependent protein kinase I Calcium/calmodulin dependent protein kinase type 1 Calcium/calmodulin-dependent protein kinase type 1 CaM K1 CaM KI CaM kinase 1 alpha CAM kinase 1 CaM kinase I alpha CaM kinase I CaM-KI CaMK 1 CAMK I CaMK1 alpha CAMK1 CAMK1 PEN CaMKI alpha CaMKI-alpha KCC1A_HUMAN MGC120317 MGC120318 |
Images
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Fig1:
Western blot analysis of CaMKI on different lysates with Rabbit anti-CaMKI antibody (HA722544) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: SH-SY5Y cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: PC-12 cell lysate (20 µg/Lane) Lane 5: Human brain tissue lysate (30 µg/Lane) Lane 6: Mouse brain tissue lysate (30 µg/Lane) Lane 7: Rat brain tissue lysate (30 µg/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722544) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of NIH/3T3 cells labeling CaMKI with Rabbit anti-CaMKI antibody (HA722544) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CaMKI antibody (HA722544) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunocytochemistry analysis of PC-12 cells labeling CaMKI with Rabbit anti-CaMKI antibody (HA722544) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CaMKI antibody (HA722544) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.