CD52 Recombinant Rabbit Monoclonal Antibody [JE37-24]
cat.: HA722545
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE37-24 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 8 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse CD52 aa 1-47 / 74. |
| Positive control: | Mouse spleen tissue lysate, mouse thymus tissue lysate, mouse spleen cells. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB FC |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q64389 Mouse |
| Alternative names: | Cambridge pathology 1 antigen CAMPATH 1 CAMPATH 1 antigen CAMPATH-1 antigen CD 52 CD52 CD52 antigen CD52 molecule CD52_HUMAN CDw52 CDW52 antigen Epididymal secretory protein E5 Epididymis secretory sperm binding protein Li 171mP HE 5 He5 Human epididymis-specific protein 5 |
Images
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Fig1:
Western blot analysis of CD52 on different lysates with Rabbit anti-CD52 antibody (HA722545) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Mouse thymus tissue lysate Lane 3: Mouse liver tissue lysate (negative) Lane 4: Mouse kidney tissue lysate (negative) Lane 5: Mouse brain tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 8 kDa Observed band size: 16 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722545) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Flow cytometric analysis of mouse spleen cells labelling CD52 (HA722545). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.