GDF15 Recombinant Rabbit Monoclonal Antibody [JE39-90]
cat.: HA722551
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE39-90 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GDF15 aa 241-290 / 308. |
| Positive control: | HepG2 cell lysate, SK-MEL-28 cell lysate, LNCaP cell lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: Q99988 Human |
| Alternative names: | GDF 15 GDF-15 Gdf15 GDF15_HUMAN Growth differentiation factor 15 Growth/differentiation factor 15 Macrophage inhibitory cytokine 1 MIC 1 Mic-1 MIC1 NAG 1 NAG-1 NAG1 NRG 1 NRG-1 NRG1 NSAID (nonsteroidal anti inflammatory drug) activated protein 1 NSAID NSAID regulated protein 1 NSAID-activated gene 1 protein NSAID-regulated gene 1 protein PDF PLAB Placental bone morphogenetic protein Placental bone morphogenic protein Placental TGF beta Placental TGF-beta Prostate differentiation factor PTGF beta PTGFB |
Images
|
Fig1:
Western blot analysis of GDF15 on different lysates with Rabbit anti-GDF15 antibody (HA722551) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: SK-MEL-28 cell lysate Lane 3: LNCaP cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 34/12.5 kDa Exposure time: 25 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722551) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.