PITX3 Recombinant Rabbit Monoclonal Antibody [JE41-12]
cat.: HA722554
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE41-12 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PITX3 aa 1-50 / 302. |
| Positive control: | HEK-293 cell lysate, SH-SY5Y cell lysate, C6 cell lysate, HeLa cell lysate, Mouse brain tissue lysate, Mouse lung tissue lysate, Mouse spleen tissue lysate, Rat brain tissue lysate, Rat lung tissue lysate, Rat spleen tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O75364 Human | O35160 Mouse | P81062 Rat |
| Alternative names: | Homeobox protein PITX 3 Homeobox protein PITX3 MGC12766 Paired like homeodomain transcription factor 3 Paired-like homeodomain transcription factor 3 Pituitary homeobox 3 PITX 3 Pitx3 PITX3_HUMAN PTX 3 PTX3 |
Images
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Fig1:
Western blot analysis of PITX3 on different lysates with Rabbit anti-PITX3 antibody (HA722554) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 µg/Lane) Lane 2: SH-SY5Y cell lysate (20 µg/Lane) Lane 3: C6 cell lysate (20 µg/Lane) Lane 4: HeLa cell lysate (20 µg/Lane) Lane 5: Mouse brain tissue lysate (40 µg/Lane) Lane 6: Mouse lung tissue lysate (40 µg/Lane) Lane 7: Mouse spleen tissue lysate (40 µg/Lane) Lane 8: Rat brain tissue lysate (40 µg/Lane) Lane 9: Rat lung tissue lysate (40 µg/Lane) Lane 10: Rat spleen tissue lysate (40 µg/Lane) Predicted band size: 32 kDa Observed band size: 35 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722554) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.