SAHH Recombinant Rabbit Monoclonal Antibody [JE41-31]
cat.: HA722555
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE41-31 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SAHH aa 351-400 / 432. |
| Positive control: | HeLa cell lysate, A549 cell lysate, HepG2 cell lysate, Jurkat cell lysate, Mouse liver tissue lysate, Mouse pancreas tissue lysate, Rat kidney tissue lysate, mouse lung tissue, rat lung tissue. |
| Subcellular location: | Cytoplasm, Melanosome, Nucleus, Endoplasmic reticulum. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P23526 Human | P50247 Mouse | P10760 Rat |
| Alternative names: | Adenosylhomocysteinase AdoHcyase ahcY S adenosyl L homocysteine hydrolase S adenosylhomocysteine hydrolase S-adenosyl-L-homocysteine hydrolase SAHH SAHH_HUMAN |
Images
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Fig1:
Western blot analysis of SAHH on different lysates with Rabbit anti-SAHH antibody (HA722555) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: HepG2 cell lysate Lane 4: Jurkat cell lysate Lane 5: Mouse liver tissue lysate Lane 6: Mouse pancreas tissue lysate Lane 7: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 48 kDa Observed band size: 45 kDa Exposure time: 1 minute 34 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722555) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-SAHH antibody (HA722555) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722555) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-SAHH antibody (HA722555) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722555) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.