MCT1 Recombinant Rabbit Monoclonal Antibody [JE41-72]
cat.: HA722556
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE41-72
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 54 kDa
Isotype: IgG
Immunogen: Recombinant protein.
Positive control: HeLa cell lysate, HepG2 cell lysate, Jurkat cell lysate, 293T cell lysate, BxPC-3 cell lysate, AGS cell lysate.
Subcellular location: Cell membrane, Basolateral cell membrane, Apical cell membrane.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P53985 Human
Alternative names: FLJ36745 HHF7 MCT 1 MCT MGC44475 Monocarboxylate transporter 1 Monocarboxylate transporter Monocarboxylate transporter isoform 1 Monocarboxylic acid transporter 1 MOT1_HUMAN Slc16a1 SLC16A1 protein Solute carrier family 16 (monocarboxylic acid transporters) member 1 Solute carrier family 16 member 1 (monocarboxylic acid transporter 1) Solute carrier family 16 member 1
Images
HA722556_1.jpg Fig1: Western blot analysis of MCT1 on different lysates with Rabbit anti-MCT1 antibody (HA722556) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: 293T cell lysate
Lane 5: BxPC-3 cell lysate
Lane 6: AGS cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 54 kDa
Observed band size: 50 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722556) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.