Dopamine beta Hydroxylase Recombinant Rabbit Monoclonal Antibody [JE43-56]
cat.: HA722558
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE43-56 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Dopamine beta Hydroxylase aa 111-260 / 617. |
| Positive control: | SH-SY5Y cell lysate. |
| Subcellular location: | Cytoplasmic vesicle, secretory vesicle membrane, secretory vesicle, chromaffin granule membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P09172 Human |
| Alternative names: | dbh DBM Dopamine beta hydroxylase Dopamine beta monooxygenase Dopamine beta-hydroxylase (dopamine beta-monooxygenase) Dopamine beta-monooxygenase DOPO_HUMAN OTTHUMP00000022501 Soluble dopamine beta-hydroxylase |
Images
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Fig1:
Western blot analysis of Dopamine beta Hydroxylase on different lysates with Rabbit anti-Dopamine beta Hydroxylase antibody (HA722558) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: HeLa cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 69 kDa Observed band size: 69 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722558) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.