CD97 Recombinant Rabbit Monoclonal Antibody [JE57-33]
cat.: HA722563
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE57-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 92 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD97 aa 400-552. |
| Positive control: | MDA-MB-231 cell lysate, HeLa cell lysate, J774A.1 cell lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P48960 Human | Q9Z0M6 Mouse |
| Alternative names: | ADGRE5 Adhesion G protein-coupled receptor E5 CD 97 CD_antigen=CD97 CD97 CD97 antigen CD97 antigen subunit beta CD97 molecule CD97_HUMAN Leukocyte antigen CD97 Seven span transmembrane protein Seven transmembrane helix receptor Seven transmembrane heterodimeric receptor associated with inflammation TM7LN 1 TM7LN1 |
Images
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Fig1:
Western blot analysis of CD97 on different lysates with Rabbit anti-CD97 antibody (HA722563) at 1/1,000 dilution. Lane 1: MDA-MB-231 cell lysate Lane 2: HeLa cell lysate Lane 3: J774A.1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 92 kDa Observed band size: 75 kDa Exposure time: Lane 1-2: 20 seconds; Lane 3: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722563) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.