Phospho-AMPK alpha 2 (S491) Recombinant Rabbit Monoclonal Antibody [JE59-49]
cat.: HA722567
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE59-49 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Ser491 of Human AMPK alpha 2. |
| Positive control: | HEK-293 cell lysate. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P54646 Human |
| Alternative names: | 5'-AMP-activated protein kinase catalytic subunit alpha-2 AAPK2_HUMAN ACACA kinase Acetyl CoA carboxylase kinase Acetyl-CoA carboxylase kinase AMPK alpha 2 chain AMPK subunit alpha-2 AMPK2 AMPKa2 AMPKalpha2 HMGCR kinase Hydroxymethylglutaryl CoA reductase kinase Hydroxymethylglutaryl-CoA reductase kinase PRKAA PRKAA2 Protein kinase AMP activated alpha 2 catalytic subunit Protein kinase AMP activated catalytic subunit alpha 2 |
Images
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Fig1:
Western blot analysis of Phospho-AMPK alpha 2 (S491) on different lysates with Rabbit anti-Phospho-AMPK alpha 2 (S491) antibody (HA722567) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HEK-293 cell lysate, the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 2 minutes 20 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722567) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.