ADH4 Recombinant Rabbit Monoclonal Antibody [JE62-24]
cat.: HA722570
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE62-24 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 40 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human ADH4 aa 331-380 / 380. |
| Positive control: | HeLa cell lysate, Human liver tissue lysate, human kidney tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P08319 Human |
| Alternative names: | Ac1002 ADH 4 ADH-1 ADH-2 ADH2 Adh4 ADH4_HUMAN Alcohol dehydrogenase 4 (class II) pi polypeptide Alcohol dehydrogenase 4 Alcohol dehydrogenase class II pi chain Alcohol dehydrogenase pi isozyme ADH class II Aldehyde reductase Class II alcohol dehydrogenase 4 pi subunit |
Images
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Fig1:
Western blot analysis of ADH4 on different lysates with Rabbit anti-ADH4 antibody (HA722570) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Human liver tissue lysate (40 µg/Lane) Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: Lane 1: 1 minute; Lane 2: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722570) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-ADH4 antibody (HA722570) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722570) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.