Nck-2 Recombinant Rabbit Monoclonal Antibody [JE63-36]
cat.: HA722573
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JE63-36 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Nck-2 aa 51-100 / 380. |
| Positive control: | A431 cell lysate, SK-MEL-28 cell lysate, HDLM-2 cell lysate, Caco-2 cell lysate, THP-1 cell lysate, A431, human testis tissue. |
| Subcellular location: | Cytoplasm, Endoplasmic reticulum. |
| Recommended Dilutions:
WB IF-Cell IHC-P IF-Tissue |
1:1,000 1:100 1:1,000 1:200 |
| Uniprot #: | SwissProt: O43639 Human |
| Alternative names: | Cytoplasmic protein NCK2 GRB4 Growth factor receptor bound protein 4 NCK adaptor protein 2 Nck-2 NCK2 NCK2_HUMAN NCKbeta Non-catalytic region of tyrosine kinase adaptor protein 2 Noncatalytic region of tyrosine kinase beta SH2/SH3 adaptor protein NCK-beta |
Images
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Fig1:
Western blot analysis of Nck-2 on different lysates with Rabbit anti-Nck-2 antibody (HA722573) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SK-MEL-28 cell lysate Lane 3: HDLM-2 cell lysate Lane 4: Caco-2 cell lysate Lane 5: THP-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 46 kDa Exposure time: 1 minute 34 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722573) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of A431 cells labeling Nck-2 with Rabbit anti-Nck-2 antibody (HA722573) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Nck-2 antibody (HA722573) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Nck-2 antibody (HA722573) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722573) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.