| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE63-70 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human RASSF2 aa 277-326 / 326. |
| Positive control: | Raji cell lysate, Jurkat cell lysate, SH-SY5Y cell lysate, U-937 cell lysate. |
| Subcellular location: | Nucleus, Cytoplasm, Chromosome, centromere, kinetochore. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P50749 Human |
| Alternative names: | CENP 34 Centromere protein 34 Ras association (RalGDS/AF 6) domain family 2 Ras association (RalGDS/AF-6) domain family member 2 Ras association domain containing protein 2 Ras association domain-containing protein 2 RASF2_HUMAN RASFADIN RASSF2 RP4-599I11.1 |
|
Fig1:
Western blot analysis of RASSF2 on different lysates with Rabbit anti-RASSF2 antibody (HA722575) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: Jurkat cell lysate Lane 3: SH-SY5Y cell lysate Lane 4: U-937 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722575) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |