RASSF2 Recombinant Rabbit Monoclonal Antibody [JE63-70]
cat.: HA722575
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE63-70
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 38 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human RASSF2 aa 277-326 / 326.
Positive control: Raji cell lysate, Jurkat cell lysate, SH-SY5Y cell lysate, U-937 cell lysate.
Subcellular location: Nucleus, Cytoplasm, Chromosome, centromere, kinetochore.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P50749 Human
Alternative names: CENP 34 Centromere protein 34 Ras association (RalGDS/AF 6) domain family 2 Ras association (RalGDS/AF-6) domain family member 2 Ras association domain containing protein 2 Ras association domain-containing protein 2 RASF2_HUMAN RASFADIN RASSF2 RP4-599I11.1
Images
HA722575_1.jpg Fig1: Western blot analysis of RASSF2 on different lysates with Rabbit anti-RASSF2 antibody (HA722575) at 1/1,000 dilution.

Lane 1: Raji cell lysate
Lane 2: Jurkat cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: U-937 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 38 kDa
Observed band size: 38 kDa

Exposure time: 1 minute; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722575) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.