PLSCR3 Recombinant Rabbit Monoclonal Antibody [JE63-89]
cat.: HA722577
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE63-89
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 32 kDa
Isotype: IgG
Immunogen: Recombinant protein within
Positive control: HeLa cell lysate, A549 cell lysate, HepG2 cell lysate, Mouse brain tissue lysate, Mouse kidney tissue lysate, Rat brain tissue lysate.
Subcellular location: Mitochondrion membrane, Mitochondrion inner membrane, Nucleus.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q9NRY6 Human | Q9JIZ9 Mouse | Q6QBQ4 Rat
Alternative names: Ca(2+)-dependent phospholipid scramblase 3 Phospholipid scramblase 3 PL scramblase 3 PLS3 PLS3_HUMAN Plscr3
Images
HA722577_1.jpg Fig1: Western blot analysis of PLSCR3 on different lysates with Rabbit anti-PLSCR3 antibody (HA722577) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Mouse brain tissue lysate
Lane 5: Mouse kidney tissue lysate
Lane 6: Rat brain tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 32 kDa
Observed band size: 32 kDa

Exposure time: 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722577) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.