Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE63-89 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 32 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within |
Positive control: | HeLa cell lysate, A549 cell lysate, HepG2 cell lysate, Mouse brain tissue lysate, Mouse kidney tissue lysate, Rat brain tissue lysate. |
Subcellular location: | Mitochondrion membrane, Mitochondrion inner membrane, Nucleus. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: Q9NRY6 Human | Q9JIZ9 Mouse | Q6QBQ4 Rat |
Alternative names: | Ca(2+)-dependent phospholipid scramblase 3 Phospholipid scramblase 3 PL scramblase 3 PLS3 PLS3_HUMAN Plscr3 |
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Fig1:
Western blot analysis of PLSCR3 on different lysates with Rabbit anti-PLSCR3 antibody (HA722577) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: HepG2 cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Mouse kidney tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722577) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |