SFT Recombinant Rabbit Monoclonal Antibody [JE64-46]
cat.: HA722578
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JE64-46 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human SFT aa 98-147 / 147. |
| Positive control: | Jurkat cell lysate, K-562 cell lysate, Raji cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, human kidney tissue, human thyroid gland carcinoma tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IP |
1:1,000 1:1,000-1:2,000 1-2μg/sample |
| Uniprot #: | SwissProt: P51668 Human | P61080 Mouse | D3ZDK2 Rat |
| Alternative names: | E2(17)KB1 SFT Stimulator of Fe transport UB2D1_HUMAN UBC 4/5 UBC4/5 UBC4/5 homolog UBC4/5, S. cerevisiae, homolog of UBCH 5 UBCH 5A UbcH5 UBCH5A Ube2d1 Ubiquitin carrier protein Ubiquitin carrier protein D1 Ubiquitin conjugating enzyme E2 17 kDa 1 Ubiquitin conjugating enzyme E2 D1 Ubiquitin conjugating enzyme E2D 1 (UBC4/5 homolog yeast) Ubiquitin conjugating enzyme E2D 1 Ubiquitin conjugating enzyme UBCH5A Ubiquitin protein ligase D1 Ubiquitin-conjugating enzyme E2 D1 Ubiquitin-conjugating enzyme E2(17)KB 1 Ubiquitin-conjugating enzyme E2-17 kDa 1 Ubiquitin-protein ligase D1 |
Images
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Fig1:
Western blot analysis of SFT on different lysates with Rabbit anti-SFT antibody (HA722578) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: K-562 cell lysate Lane 3: Raji cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse kidney tissue lysate Lane 7: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 17 kDa Observed band size: 15 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722578) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
SFT was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA722578 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722578 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: HA722578 IP in Jurkat cell lysate Lane 3: Rabbit IgG instead of HA722578 in Jurkat cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 26 seconds; ECL: K1801 |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SFT antibody (HA722578) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722578) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human thyroid gland carcinoma tissue with Rabbit anti-SFT antibody (HA722578) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722578) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.