MRas Recombinant Rabbit Monoclonal Antibody [JE64-57]
cat.: HA722579
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE64-57 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human MRas aa 159-208 / 208. |
| Positive control: | SH-SY5Y cell lysate, Neuro-2a cell lysate, U-87 MG cell lysate, C6 cell lysate, Human brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O14807 Human | O08989 Mouse | P97538 Rat |
| Alternative names: | FLJ42964 M ras M-ras Mras Muscle and microspikes Ras Muscle RAS oncogene homolog Muscle Ras oncogene homologue Muscle Ras viral oncogene homolog R ras3 R-ras3 Ras related protein MRas Ras related protein RRas3 Ras-related protein M-Ras Ras-related protein R-Ras3 RASM_HUMAN Related Ras viral oncogene homolog 3 RRas3 XRas |
Images
|
Fig1:
Western blot analysis of MRas on different lysates with Rabbit anti-MRas antibody (HA722579) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane) Lane 2: Neuro-2a cell lysate (20 µg/Lane) Lane 3: U-87 MG cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: Human brain tissue lysate (40 µg/Lane) Lane 6: Mouse brain tissue lysate (40 µg/Lane) Lane 7: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: Lane 1-5: 59 seconds; Lane 6-7: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722579) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.