TNFAIP8 Recombinant Rabbit Monoclonal Antibody [JE65-07]
cat.: HA722580
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE65-07
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 23 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human TNFAIP8 aa 1-50 / 198.
Positive control: HeLa cell lysate, K-562 cell lysate, A549 cell lysate, THP-1 cell lysate, Mouse thymus tissue lysate, Rat thymus tissue lysate.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: O95379 Human | Q921Z5 Mouse
Entrez Gene: 307428 Rat
Alternative names: GG2 1 Head and neck tumor and metastasis-related protein MDC-3.13 NDED NF-kappa-B-inducible DED-containing protein SCC S2 SCC-S2 SCCS2 TFIP8_HUMAN TNF alpha-induced protein 8 TNF induced protein TNF-induced protein GG2-1 TNFAIP 8 TNFAIP8 Tumor necrosis factor alpha induced protein 8 Tumor necrosis factor alpha-induced protein 8
Images
HA722580_1.jpg Fig1: Western blot analysis of TNFAIP8 on different lysates with Rabbit anti-TNFAIP8 antibody (HA722580) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: THP-1 cell lysate (20 µg/Lane)
Lane 5: Mouse thymus tissue lysate (40 µg/Lane)
Lane 6: Rat thymus tissue lysate (40 µg/Lane)

Predicted band size: 23 kDa
Observed band size: 19/21 kDa

Exposure time: 30 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722580) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.