| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE65-07 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human TNFAIP8 aa 1-50 / 198. |
| Positive control: | HeLa cell lysate, K-562 cell lysate, A549 cell lysate, THP-1 cell lysate, Mouse thymus tissue lysate, Rat thymus tissue lysate. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O95379 Human | Q921Z5 Mouse Entrez Gene: 307428 Rat |
| Alternative names: | GG2 1 Head and neck tumor and metastasis-related protein MDC-3.13 NDED NF-kappa-B-inducible DED-containing protein SCC S2 SCC-S2 SCCS2 TFIP8_HUMAN TNF alpha-induced protein 8 TNF induced protein TNF-induced protein GG2-1 TNFAIP 8 TNFAIP8 Tumor necrosis factor alpha induced protein 8 Tumor necrosis factor alpha-induced protein 8 |
|
Fig1:
Western blot analysis of TNFAIP8 on different lysates with Rabbit anti-TNFAIP8 antibody (HA722580) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: K-562 cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (20 µg/Lane) Lane 4: THP-1 cell lysate (20 µg/Lane) Lane 5: Mouse thymus tissue lysate (40 µg/Lane) Lane 6: Rat thymus tissue lysate (40 µg/Lane) Predicted band size: 23 kDa Observed band size: 19/21 kDa Exposure time: 30 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722580) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |