Nesprin3 Recombinant Rabbit Monoclonal Antibody [JE65-22]
cat.: HA722581
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE65-22 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 112 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Nesprin3 aa 6776-925 / 975. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, HEK-293 cell lysate, LNCaP cell lysate. |
| Subcellular location: | Nucleus outer membrane, Nucleus envelope, Rough endoplasmic reticulum. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q6ZMZ3 Human |
| Alternative names: | C14orf49 Chromosome 14 open reading frame 49 FLJ25605 MGC75267 Nesp3 Nesprin 3 Nesprin-3 NET53 Nuclear Envelope Spectrin Repeat Protein 3 Spectrin Repeat Containing, Nuclear Envelope Family Member 3 SYNE3 SYNE3_HUMAN |
Images
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Fig1:
Western blot analysis of Nesprin3 on different lysates with Rabbit anti-Nesprin3 antibody (HA722581) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HEK-293 cell lysate Lane 4: LNCaP cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 112 kDa Observed band size: 100 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722581) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.