Phospho-CDK1 (T161) + CDK2/CDK3 (T160) Recombinant Rabbit Monoclonal Antibody [JE75-37]
cat.: HA722582
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, Dot Blot |
| Clonality: | Monoclonal |
| Clone number: | JE75-37 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 34 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Thr160 of human CDK2. |
| Positive control: | HeLa treated with 100ng/mL Nocodazole for 18 hours cell lysate. |
| Subcellular location: | Nucleus, Cytoplasm, Mitochondrion. |
| Recommended Dilutions:
WB Dot Blot |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P24941 Human | P06493 Human | Q00526 Human |
| Alternative names: | Cdc 2 Cdc2 CDC28A CDK 1 CDK1 CDK1_HUMAN CDKN1 CELL CYCLE CONTROLLER CDC2 Cell division control protein 2 Cell division control protein 2 homolog Cell division cycle 2 G1 to S and G2 to M Cell division protein kinase 1 Cell Divsion Cycle 2 Protein Cyclin Dependent Kinase 1 Cyclin-dependent kinase 1 DKFZp686L20222 MGC111195 p34 Cdk1 p34 protein kinase P34CDC2 Cdc2 related protein kinase cdc2-related protein kinase CDC28 CDC2A Cdk 2 CDK2 CDK2_HUMAN CDKN2 Cell devision kinase 2 Cell division protein kinase 2 Cyclin dependent kinase 2 cyclin dependent kinase 2-alpha Cyclin-dependent kinase 2 kinase Cdc2 MPF p33 protein kinase p33(CDK2) Cdk 3 Cdk3 CDK3_HUMAN CDKN3 Cell division kinase 3 Cell division protein kinase 3 Cyclin dependent kinase 3 Cyclin-dependent kinase 3 OTTHUMP00000206828 p36 |
Images
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Fig1:
Western blot analysis of Phospho-CDK1 (T161) + CDK2/CDK3 (T160) on different lysates with Rabbit anti-Phospho-CDK1 (T161) + CDK2/CDK3 (T160) antibody (HA722582) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 100ng/mL Nocodazole for 18 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722582) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Dot blot analysis of Phospho-CDK1 (T161) + CDK2/CDK3 (T160) on different proteins with Rabbit anti-Phospho-CDK1 (T161) + CDK2/CDK3 (T160) antibody (HA722582) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution for 1 hour at room temperature. Lane 1: Unmodified CDK1 (negative) Lane 2: Phospho-CDK1(T161) (positive) Lane 3: Unmodified CDK2 (negative) Lane 4: Phospho-CDK2(T160) (positive) Proteins loading: 25ng, 5ng, 1ng; Blocking and dilution buffer: 5% NFDM/TBST; Exposure time: 1 minute; ECL: K1801. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.