Bcl G/BCL2L14 Recombinant Rabbit Monoclonal Antibody [JE77-24]
cat.: HA722583
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE77-24
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 37 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Bcl G/BCL2L14 aa 1-250 / 327.
Positive control: HeLa cell lysate, K-562 cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q9BZR8 Human | Q9CPT0 Mouse | Q6AYK4 Rat
Alternative names: Apoptosis facilitator Bcl 2 like protein 14 Apoptosis regulator BCL G Bcl2 L 14 BCL2 like 14 (apoptosis facilitator) BCL2 like 14 BCL2L14 BCLG testicular tissue protein Li 26
Images
HA722583_1.jpg Fig1: Western blot analysis of Bcl G/BCL2L14 on different lysates with Rabbit anti-Bcl G/BCL2L14 antibody (HA722583) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: C6 cell lysate
Lane 7: PC-12 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 37 kDa
Observed band size: 45 kDa

Exposure time: 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722583) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.