Biotin Conjugated Human Adiponectin Recombinant Rabbit Monoclonal Antibody [PSH05-32]
cat.: HA722595B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Cynomolgus monkey |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH05-32 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Adiponectin aa 19-244 (Q15848). |
| Positive control: | Recombinant standard Human Adiponectin protein (HA210935). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH05-31] to Human Adiponectin (Capture) (HA722260) and Recombinant Human Adiponectin protein (HA210935) as the standard. The reference range value is 39.06-5000pg/ml. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q15848 Human |
| Alternative names: | 30 kDa adipocyte complement related protein 30 kDa adipocyte complement-related protein ACDC Acrp 30 ACRP30 ADIPO_HUMAN Adipocyte Adipocyte C1q and collagen domain containing protein Adipocyte complement related 30 kDa protein Adipocyte complement related protein of 30 kDa Adipocyte complement-related 30 kDa protein adipocyte-specific secretory protein Adiponectin Adiponectin precursor adiponectin, C1Q and collagen domain containing Adipoq Adipose most abundant gene transcript 1 Adipose most abundant gene transcript 1 protein Adipose specific collagen like factor ADIPQTL1 ADPN APM 1 apM-1 APM1 C1q and collagen domain-containing protein GBP 28 GBP28 Gelatin binding protein Gelatin binding protein 28 gelatin-binding protein 28 Gelatin-binding protein OTTHUMP00000210047 |
Images
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Fig1:
Sandwich ELISA analysis of Human Adiponectin matched pair antibodies Capture: HA722260, Human Adiponectin Rabbit mAb [PSH05-31] Detector: HA722261, Human Adiponectin Rabbit mAb [PSH05-32] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722260) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Adiponectin protein (HA210935) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA722261, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.