Biotin Conjugated Mouse IFN beta Recombinant Rabbit Monoclonal Antibody [PSH04-73]
cat.: HA722605B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH04-73 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse IFN beta aa 22-182 (P01575). |
| Positive control: | Recombinant Mouse IFN beta protein (HA210789). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH04-72] to Mouse IFN beta antibody (Capture) (HA722172) and Recombinant Mouse IFN beta protein (HA210789) as the standard. The reference range value is 62.5-2,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P01575 Mouse |
| Alternative names: | beta-interferon Fibroblast interferon IFB IFF IFN beta IFN-beta IFNB 1 IFNB IFNB_HUMAN IFNB1 Interferon beta 1 fibroblast Interferon beta Interferon beta precursor MGC96956 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse IFN beta matched pair antibodies Capture: HA722172, Mouse IFN beta Rabbit mAb [PSH04-72] Detector: HA722173, Mouse IFN beta Rabbit mAb [PSH04-73] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722172) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Recombinant Mouse IFN beta protein (HA210789) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA722173, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.