SPARC Recombinant Rabbit Monoclonal Antibody [PSH06-23]
cat.: HA722655
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH06-23 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SPARC aa 1-303. |
| Positive control: | A375 cell lysate, SH-SY5Y cell lysate, MG-63 cell lysate, RWPE-1 cell lysate. |
| Subcellular location: | Secreted, extracellular space, extracellular matrix, basement membrane. |
| Recommended Dilutions:
WB |
:1,000 |
| Uniprot #: | SwissProt: P09486 Human |
| Alternative names: | AA517111 Basement membrane protein 40 Basement-membrane protein 40 BM 40 BM-40 BM40 Cysteine rich protein hm:zeh0062 MGC128090 OI17 ON Osteonectin Secreted acidic cystein rich glycoprotein Secreted protein acidic and cysteine rich Secreted protein acidic and rich in cysteine Secreted protein acidic cysteine rich (osteonectin) Secreted protein acidic cysteine rich SPARC SPRC SPRC_HUMAN |
Images
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Fig1:
Western blot analysis of SPARC on different lysates with Rabbit anti-SPARC antibody (HA722655) at 1/1,000 dilution. Lane 1: A375 cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: MG-63 cell lysate Lane 4: RWPE-1 cell lysate Lane 5: MCF7 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722655) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.