Phospho-PLC-gamma-1 (Y783) Recombinant Rabbit Monoclonal Antibody [PSH06-29]
cat.: HA722661
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH06-29 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 149 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phosphopeptide corresponding to residues surrounding Tyr783 of human PLC-gamma-1. |
| Positive control: | A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, NIH/3T3 starved overnight then add 100ng/mL PDGF for 5 minutes cell lysate. |
| Subcellular location: | Cell projection, lamellipodium, ruffle. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P19174 Human | Q62077 Mouse | P10686 Rat |
| Alternative names: | 1 phosphatidyl D myo inositol 4 5 bisphosphate 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 Inositoltrisphosphohydrolase Monophosphatidylinositol phosphodiesterase NCKAP3 Phosphatidylinositol phospholipase C Phosphoinositidase C Phosphoinositide phospholipase C Phosphoinositide phospholipase C-gamma-1 Phospholipase C 148 Phospholipase C gamma 1 Phospholipase C-gamma-1 Phospholipase C-II PLC gamma 1 PLC II PLC-148 PLC-gamma-1 PLC-II PLC1 PLC148 Plcg1 PLCG1_HUMAN PLCgamma1 |
Images
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Fig1:
Western blot analysis of Phospho-PLC-gamma-1 (Y783) on different lysates with Rabbit anti-Phospho-PLC-gamma-1 (Y783) antibody (HA722661) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 starved overnight then add 100ng/mL PDGF for 5 minutes cell lysate Lane 5: A431 starved overnight then add 20ng/mL EGF for 5 minutes cell lysate, then the membrane treated with λpp for 1 hour Lysates/proteins at 20 µg/Lane. Predicted band size: 149 kDa Observed band size: 149 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722661) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.