CD133 Recombinant Rabbit Monoclonal Antibody [PSH06-48]
cat.: HA722667
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IHC-P, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | PSH06-48 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 97 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within |
| Positive control: | Mouse kidney tissue lysate, mouse colon tissue. |
| Subcellular location: | Endoplasmic reticulum. Plasma membrane. Cell projection. |
| Recommended Dilutions:
WB IHC-P IHC-Fr |
1:1,000 1:200 1:100 |
| Uniprot #: | SwissProt: O54990 Mouse |
| Alternative names: | AC133 Antigen AC133 CD133 CORD12 Hematopoietic stem cell antigen hProminin MCDR2 MSTP061 OTTHUMP00000217744 OTTHUMP00000217745 OTTHUMP00000217746 PROM1 PROM1_HUMAN Prominin I Prominin like 1 Prominin like protein 1 precursor Prominin mouse like 1 Prominin-1 Prominin-like protein 1 Prominin1 PROML1 RP41 STGD4 |
Images
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Fig1:
Western blot analysis of CD133 on different lysates with Rabbit anti-CD133 antibody (HA722667) at 1/1,000 dilution. Lane 1: Mouse kidney tissue lysate Lane 2: Mouse kidney tissue lysate treated with deglycosylation Lysates/proteins at 20 µg/Lane. Predicted band size: 97 kDa Observed band size: 120/97 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722667) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-CD133 antibody (HA722667) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722667) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunofluorescence analysis of frozen mouse colon tissue with Rabbit anti-CD133 antibody (HA722667) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA722667, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.