TNFRSF14/HVEM Recombinant Rabbit Monoclonal Antibody [PSH06-41]
cat.: HA722676
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH06-41 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TNFRSF14 aa 39-202. |
| Positive control: | Recombinant standard Human TNFRSF14 protein (HA210556). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH06-42] to Human TNFRSF14 antibody (Detecor) (HA722677) and recombinant standard Human TNFRSF14 protein (HA210556) as the standard. The reference range value is 10.3-2,500 pg/ml. |
| Uniprot #: | SwissProt: Q92956 Human |
| Alternative names: | HVEML ATAR CD270 CD40 like protein precursor Herpes virus entry mediator A Herpesvirus entry mediator A Herpesvirus entry mediator Herpesvirus entry mediator ligand HveA HVEM HVEM L LIGHT LIGHTR TNFRSF14 TNFSF 14 TNR14_HUMAN TR2 Tumor necrosis factor receptor like gene2 Tumor necrosis factor receptor superfamily member 14 Tumor necrosis factor receptor superfamily member 14 precursor Tumor necrosis factor receptor-like 2 |
Images
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Fig1:
Sandwich ELISA analysis of Human HVEM matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722676) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant standard Human TNFRSF14 protein (HA210556) starting from 4,500 pg/ml to 0 pg/ml and detect antibody (HA722677, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native HVEM in human serum samples. The concentrations of HVEM were measured in triplicates, interpolated from the HVEM standard curve and corrected for sample dilution. Undiluted samples are human serum 10%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=3). The mean HVEM concentration was determined to be 5,867 pg/ml in human serum. |
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Fig3:
Interpolated concentrations of native HVEM in Raji and HL-60 extract samples based on a 1,000 µg/ml extract load. The concentrations of HVEM were measured in duplicates, interpolated from the HVEM standard curve and corrected for sample dilution. Undiluted samples are Raji extract 13% and HL-60 extract 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HVEM concentration was determined to be 3,332 pg/ml in Raji extract and 1,901 pg/ml in HL-60 extract. |
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Fig4:
Interpolated concentrations of spiked HVEM in human cell culture media samples. The concentrations of HVEM were measured in duplicates, interpolated from the HVEM standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.