Biotin Conjugated Human IFNGR1 Recombinant Rabbit Monoclonal Antibody [PSH06-44]
cat.: HA722681B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH06-44 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IFNGR1 aa 18-245. |
| Positive control: | Recombinant standard Human IFNGR1 protein (HA210514). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH06-43] to Human IFNGR1 antibody (Capture) (HA722679) and recombinant standard Human IFNGR1 protein (HA210514) as the standard. The reference range value is 41.2-10,000 pg/ml. |
| Uniprot #: | SwissProt: P15260 Human |
| Alternative names: | Antiviral Protein Type II Antiviral protein, type 2 AVP type II AVP, type 2 CD 119 CD119 CD119 antigen CDw119 FLJ45734 IFN gamma R alpha IFN gamma R IFN gamma R1 IFN-gamma receptor 1 IFN-gamma-R1 IFNG R1 IFNGR 1 IFNGR IFNGR1 Immune interferon receptor 1 Immune interferon receptor for INGR1_HUMAN Interferon gamma receptor 1 Interferon gamma receptor alpha chain Interferon gamma receptor alpha chain precursor |
Images
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Fig1:
Sandwich ELISA analysis of human IFNGR1 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA722679) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant standard Human IFNGR1 protein (HA210514) starting from 8,000 pg/ml to 0 pg/ml and detect antibody (HA722681B, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native IFNGR1 in MDA-MB-231 and MCF7 extract samples based on a 1,000 µg/ml extract load. The concentrations of IFNGR1 were measured in duplicates, interpolated from the IFNGR1 standard curve and corrected for sample dilution. Undiluted samples are MDA-MB-231 extract 50% and MCF7 extract 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IFNGR1 concentration was determined to be 2,358 pg/ml in MDA-MB-231 extract and 1,055 pg/ml in MCF7 extract. |
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Fig3:
Interpolated concentrations of spiked IFNGR1 in human cell culture media samples. The concentrations of IFNGR1 were measured in duplicates, interpolated from the IFNGR1 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.