Human Complement C5 Recombinant Rabbit Monoclonal Antibody [PSH06-46]
cat.: HA722683
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH06-46 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Complement C5 19-821. |
| Positive control: | Recombinant standard Human Complement C5 protein (HA210636). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH06-45] to Human Complement C5 antibody (Capture) (HA722682) and recombinant standard Human Complement C5 protein (HA210636). he reference range value is 111-9,000pg/ml. |
| Uniprot #: | SwissProt: P01031 Human |
| Alternative names: | Anaphylatoxin C5a analog C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4 C5 C5a anaphylatoxin C5a C5b CO5_HUMAN Complement C5 alpha'' chain Complement C5 Complement component C5 CPAMD4 prepro-C5 |
Images
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Fig1:
Sandwich ELISA analysis of human Complement C5 matched pair antibodies Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722682) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted human Complement C5 protein starting from 4000 pg/ml to 0 pg/ml and detect antibody (HA722683, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2: Interpolated concentration of native Complement C5 was measured in duplicate at different sample concentrations. Undiluted samples were 1:10000 serum. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Complement C5 concentration was determined to be 78.3 ug/mL in human serum. |
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Fig3:
Interpolated concentrations of native Complement C5 in human cell culture supernatant samples. Complement C5 was measured in 100% cell supernatant. The concentrations of Complement C5 were interpolated from the Complement C5 standard curves. The mean Complement C5 concentration was determined to be 5.256 ng/mL in HepG2 cell supernanant. There was no detectable signal in U-2 OS cell supernatant. |
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Fig4:
Interpolated concentrations of spiked Complement C5 in human cell culture media samples. The concentrations of Complement C5 were measured in duplicates, interpolated from the Complement C5 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.