Torsin A / DYT1 Recombinant Rabbit Monoclonal Antibody [JE01-51]
cat.: HA722686
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE01-51 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Torsin A / DYT1 aa 283-332 / 332. |
| Positive control: | HepG2 cell lysate, 293T cell lysate, A549 cell lysate, SW480 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse brain tissue lysate. |
| Subcellular location: | Endoplasmic reticulum lumen, Nucleus membrane, Cell projection, growth cone, Cytoplasmic vesicle membrane, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle, Cytoplasm, cytoskeleton. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O14656 Human |
| Alternative names: | DQ2 Dystonia 1 Dystonia 1 protein Dystonia 1 torsion Dyt1 TOR1A TOR1A_HUMAN Torsin 1A Torsin A Torsin family 1 member A Torsin family 1, member A (torsin A) Torsin-1A |
Images
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Fig1:
Western blot analysis of Torsin A / DYT1 on different lysates with Rabbit anti-Torsin A / DYT1 antibody (HA722686) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (20 µg/Lane) Lane 4: SW480 cell lysate (20 µg/Lane) Lane 5: NIH/3T3 cell lysate (20 µg/Lane) Lane 6: C6 cell lysate (20 µg/Lane) Lane 7: Mouse brain tissue lysate (40 µg/Lane) Predicted band size: 38 kDa Observed band size: 35 kDa Exposure time: 1 minute 2 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722686) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.