Lin28B Recombinant Rabbit Monoclonal Antibody [JE04-80]
cat.: HA722688
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC, IP |
| Clonality: | Monoclonal |
| Clone number: | JE04-80 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within |
| Positive control: | HepG2 cell lysate, JAR cell lysate, K-562 cell lysate, JAR. |
| Subcellular location: | Nucleus, nucleolus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell FC IP |
1:1,000 1:100 1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: Q6ZN17 Human |
| Alternative names: | CSDD 2 CSDD2 FLJ16517 Lin 28 homolog B (C. elegans) Lin 28.2 Lin-28B LIN28B LN28B_HUMAN Protein lin-28 homolog B |
Images
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Fig1:
Western blot analysis of Lin28B on different lysates with Rabbit anti-Lin28B antibody (HA722688) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate (negative) Lane 3: JAR cell lysate Lane 4: K-562 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 30 kDa Observed band size: 35 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722688) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of JAR cells labeling Lin28B with Rabbit anti-Lin28B antibody (HA722688) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Lin28B antibody (HA722688) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of JAR cells labeling Lin28B. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722688, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
Lin28B was immunoprecipitated from 0.2 mg JAR cell lysate with HA722688 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722688 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: JAR cell lysate (input) Lane 2: HA722688 IP in JAR cell lysate Lane 3: Rabbit IgG instead of HA722688 in JAR cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 8 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.