AMPK gamma 1 Recombinant Rabbit Monoclonal Antibody [JE07-47]
cat.: HA722691
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JE07-47 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human AMPK gamma 1 aa 282-331 / 331. |
| Positive control: | HeLa cell lysate, HEK-293 cell lysate, HepG2 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, Mouse heart tissue lysate, Rat heart tissue lysate. |
| Subcellular location: | Cytoplasm, cytosol, membrane, nucleoplasm, nucleus. |
| Recommended Dilutions:
WB IP |
1:1,000 1-2μg/sample |
| Uniprot #: | SwissProt: P54619 Human | O54950 Mouse | P80385 Rat |
| Alternative names: | 5' AMP activated protein kinase gamma 1 subunit 5' AMP activated protein kinase subunit gamma 1 5''-AMP-activated protein kinase subunit gamma-1 AAKG1_HUMAN AMP activated protein kinase noncatalytic gamma 1 subunit AMPK gamma 1 chain AMPK gamma1 AMPK subunit gamma-1 AMPKg MGC8666 PRKAG 1 PRKAG1 Protein kinase AMP activated gamma 1 non catalytic subunit protein kinase, AMP-activated, noncatalytic gamma-1 |
Images
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Fig1:
Western blot analysis of AMPK gamma 1 on different lysates with Rabbit anti-AMPK gamma 1 antibody (HA722691) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: HepG2 cell lysate Lane 4: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722691) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of AMPK gamma 1 on different lysates with Rabbit anti-AMPK gamma 1 antibody (HA722691) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: Mouse heart tissue lysate Lane 3: Rat heart tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722691) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
AMPK gamma 1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with HA722691 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722691 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input) Lane 2: HA722691 IP in Jurkat cell lysate Lane 3: Rabbit IgG instead of HA722691 in Jurkat cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.