CD3 zeta Recombinant Rabbit Monoclonal Antibody [JE09-37]
cat.: HA722692
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | JE09-37 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 19 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CD3 zeta aa 115-164 / 164. |
| Positive control: | Jurkat cell lysate, human spleen tissue, human thymus tissue, human tonsil tissue, rat spleen tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:1,000 1:200 |
| Uniprot #: | SwissProt: P20963 Human Entrez Gene: 25300 Rat |
| Alternative names: | 4930549J05Rik A430104F18Rik AW552088 CD247 CD247 antigen Cd247 molecule Cd3 CD3 antigen, zeta polypeptide, isoform CRA_b CD3 antigen, zeta subunit CD3-eta CD3H CD3Q Cd3z CD3Z antigen zeta polypeptide (TiT3 complex) CD3Z_HUMAN CD3zeta CD3zeta chain MGC140430 T cell receptor T3 zeta chain T cell surface glycoprotein CD3 zeta chain T-cell antigen receptor complex, zeta subunit of CD3 T-cell receptor T3 zeta chain T-cell surface glycoprotein CD3 zeta chain T3z TCR zeta chain TCRk TCRZ TCRzeta |
Images
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Fig1:
Western blot analysis of CD3 zeta on different lysates with Rabbit anti-CD3 zeta antibody (HA722692) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HeLa cell lysate (negative) Lane 3: 293T cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 19 kDa Observed band size: 16 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722692) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD3 zeta antibody (HA722692) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722692) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human thymus tissue with Rabbit anti-CD3 zeta antibody (HA722692) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722692) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD3 zeta antibody (HA722692) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722692) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CD3 zeta antibody (HA722692) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722692) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Application: IF-Tissue Species: Human Site: Spleen Sample: Paraffin-embedded section Antibody concentration: 1/200 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.