BMP2 Recombinant Rabbit Monoclonal Antibody [JE10-29]
cat.: HA722693
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE10-29 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human BMP2 aa 51-100 / 396. |
| Positive control: | Caco-2 cell lysate, Saos-2 cell lysate, LNCaP cell lysate, HeLa cell lysate, Human lung tissue lysate, Human liver tissue lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P12643 Human | P21274 Mouse | P49001 Rat |
| Alternative names: | BDA2 BMP-2 BMP-2A Bmp2 BMP2_HUMAN BMP2A Bone morphogenetic protein 2 Bone morphogenetic protein 2A |
Images
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Fig1:
Western blot analysis of BMP2 on different lysates with Rabbit anti-BMP2 antibody (HA722693) at 1/1,000 dilution. Lane 1: Caco-2 cell lysate Lane 2: Saos-2 cell lysate Lane 3: LNCaP cell lysate Lane 4: HeLa cell lysate Lane 5: Human lung tissue lysate Lane 6: Human liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 38 kDa Exposure time: 1 minute 2 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722693) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of BMP2 on different lysates with Rabbit anti-BMP2 antibody (HA722693) at 1/1,000 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat spleen tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 45 kDa Observed band size: 38 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722693) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.