AKR1C1 / AKR1C2 Recombinant Rabbit Monoclonal Antibody [JE33-84]
cat.: HA722694
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE33-84 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within |
| Positive control: | HeLa cell lysate, HepG2 cell lysate, Human liver tissue lysate, Mouse liver tissue lysate, HepG2. |
| Subcellular location: | Cytoplasm, cytosol. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:100 1:1,000 |
| Uniprot #: | SwissProt: Q04828 Human | P52895 Human |
| Alternative names: | 20 alpha (3 alpha) hydroxysteroid dehydrogenase 20-alpha-HSD 20-alpha-hydroxysteroid dehydrogenase 3-alpha-HSD3 AKR1C1 AKR1C2 Aldo-keto reductase family 1 member C1 Aldo-keto reductase family 1 member C2 Chlordecone reductase homolog HAKRC Chlordecone reductase homolog HAKRD DD-2 DD/BABP DD1 DD1/DD2 DD2 DDH DDH1 DDH2 Dihydrodiol dehydrogenase 1 Dihydrodiol dehydrogenase 1/2 Dihydrodiol dehydrogenase 2 dihydrodiol dehydrogenase isoform DD1 Dihydrodiol dehydrogenase/bile acid-binding protein HBAB Hepatic dihydrodial dehydrogenase High-affinity hepatic bile acid-binding protein Indanol dehydrogenase MBAB MGC8954 Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase Type III 3-alpha-hydroxysteroid dehydrogenase |
Images
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Fig1:
Western blot analysis of AKR1C1 / AKR1C2 on different lysates with Rabbit anti-AKR1C1 / AKR1C2 antibody (HA722694) at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: HepG2 cell lysate (10 µg/Lane) Lane 3: Human liver tissue lysate (20 µg/Lane) Lane 4: Mouse liver tissue lysate (20 µg/Lane) Predicted band size: 37 kDa Observed band size: 37 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722694) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HepG2 cells labeling AKR1C1 / AKR1C2 with Rabbit anti-AKR1C1 / AKR1C2 antibody (HA722694) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AKR1C1 / AKR1C2 antibody (HA722694) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of HepG2 cells labeling AKR1C1 / AKR1C2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA722694, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.