AKR1C1 / AKR1C2 Recombinant Rabbit Monoclonal Antibody [JE33-84]
cat.: HA722694
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, FC
Clonality: Monoclonal
Clone number: JE33-84
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 37 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human AKR1C1 aa 51-100 / 323.
Positive control: HeLa cell lysate, HepG2 cell lysate, Human liver tissue lysate, Mouse liver tissue lysate, Mouse testis tissue lysate, HepG2.
Subcellular location: Cytoplasm, cytosol.
Recommended Dilutions:
  WB
  IF-Cell
  FC
1:1,000
1:100
1:1,000
Uniprot #: SwissProt: Q04828 Human | P52895 Human
Alternative names: 20 alpha (3 alpha) hydroxysteroid dehydrogenase 20-alpha-HSD 20-alpha-hydroxysteroid dehydrogenase 3-alpha-HSD3 AKR1C1 AKR1C2 Aldo-keto reductase family 1 member C1 Aldo-keto reductase family 1 member C2 Chlordecone reductase homolog HAKRC Chlordecone reductase homolog HAKRD DD-2 DD/BABP DD1 DD1/DD2 DD2 DDH DDH1 DDH2 Dihydrodiol dehydrogenase 1 Dihydrodiol dehydrogenase 1/2 Dihydrodiol dehydrogenase 2 dihydrodiol dehydrogenase isoform DD1 Dihydrodiol dehydrogenase/bile acid-binding protein HBAB Hepatic dihydrodial dehydrogenase High-affinity hepatic bile acid-binding protein Indanol dehydrogenase MBAB MGC8954 Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase Type III 3-alpha-hydroxysteroid dehydrogenase
Images
HA722694_1.jpg Fig1: Western blot analysis of AKR1C1 / AKR1C2 on different lysates with Rabbit anti-AKR1C1 / AKR1C2 antibody (HA722694) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (10 µg/Lane)
Lane 2: HepG2 cell lysate (10 µg/Lane)
Lane 3: Human liver tissue lysate (20 µg/Lane)
Lane 4: Mouse liver tissue lysate (20 µg/Lane)

Predicted band size: 37 kDa
Observed band size: 37 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722694) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA722694_2.jpg Fig2: Western blot analysis of AKR1C1 / AKR1C2 on different lysates with Rabbit anti-AKR1C1 / AKR1C2 antibody (HA722694) at 1/2,000 dilution.

Lane 1: HepG2 cell lysate (20 µg/Lane)
Lane 2: Mouse testis tissue lysate (30 µg/Lane)
Lane 3: Mouse liver tissue lysate (30 µg/Lane)
Lane 4: 293T cell lysate (negative) (20 µg/Lane)

Predicted band size: 37 kDa
Observed band size: 37 kDa

Exposure time: 12 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722694) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA722694_3.jpg Fig3: Immunocytochemistry analysis of HepG2 cells labeling AKR1C1 / AKR1C2 with Rabbit anti-AKR1C1 / AKR1C2 antibody (HA722694) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AKR1C1 / AKR1C2 antibody (HA722694) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA722694_4.jpg Fig4: Flow cytometric analysis of HepG2 cells labeling AKR1C1 / AKR1C2.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA722694, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.