IDH1 Recombinant Rabbit Monoclonal Antibody [JE34-27]
cat.: HA722696
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | JE34-27 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human IDH1 aa 51-100 / 414. |
| Positive control: | HeLa cell lysate, Raji cell lysate, HepG2 cell lysate, Neuro-2a cell lysate, C6 cell lysate, Mouse kidney tissue lysate, Rat kidney tissue lysate, human prostate cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue. |
| Subcellular location: | Cytoplasm, cytosol, Peroxisome. |
| Recommended Dilutions:
WB IHC-P IP |
1:1,000-1:2,000 1:3,000 1-2μg/sample |
| Uniprot #: | SwissProt: O75874 Human | O88844 Mouse | P41562 Rat |
| Alternative names: | Cytosolic NADP isocitrate dehydrogenase Cytosolic NADP-isocitrate dehydrogenase Epididymis luminal protein 216 Epididymis secretory protein Li 26 HEL-216 HEL-S-26 ICDH IDCD IDH IDH1 IDHC_HUMAN IDP IDPC Isocitrate dehydrogenase (NADP(+)) 1 cytosolic Isocitrate dehydrogenase [NADP] cytoplasmic Isocitrate dehydrogenase 1 (NADP+) soluble NADP dependent isocitrate dehydrogenase cytosolic NADP dependent isocitrate dehydrogenase peroxisomal NADP(+)-specific ICDH Oxalosuccinate decarboxylase PICD |
Images
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Fig1:
Western blot analysis of IDH1 on different lysates with Rabbit anti-IDH1 antibody (HA722696) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Raji cell lysate Lane 3: HepG2 cell lysate Lane 4: Neuro-2a cell lysate Lane 5: C6 cell lysate Lane 6: Mouse kidney tissue lysate Lane 7: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722696) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of IDH1 on different lysates with Rabbit anti-IDH1 antibody (HA722696) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-IDH1 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 47 kDa Observed band size: 47 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722696) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-IDH1 antibody (HA722696) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722696) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-IDH1 antibody (HA722696) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722696) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-IDH1 antibody (HA722696) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722696) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-IDH1 antibody (HA722696) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722696) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
IDH1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA722696 at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using HA722696 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA722696 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of HA722696 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 8 seconds; ECL: K1802 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.