HES5 Recombinant Rabbit Monoclonal Antibody [JE35-96]
cat.: HA722699
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE35-96 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 18 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human HES5 aa 1-166 / 166. |
| Positive control: | HEK-293 cell lysate, U-87 MG cell lysate, C6 cell lysate, Mouse kidney tissue lysate, Rat liver tissue lysate, Rat kidney tissue lysate, human glioblastoma tissue, human small intestine tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q5TA89 Human | P70120 Mouse | Q03062 Rat |
| Alternative names: | bHLHb38 Class B basic helix-loop-helix protein 38 Hairy and enhancer of split 5 Hairy/Enhancer of spli, Drosophila, homolog of, 5 HES 5 hes family bHLH transcription factor 5 Hes5 HES5 protein HES5_HUMAN MGI:104876 Transcription factor HES-5 |
Images
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Fig1:
Western blot analysis of HES5 on different lysates with Rabbit anti-HES5 antibody (HA722699) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: U-87 MG cell lysate Lane 3: C6 cell lysate Lane 4: Mouse kidney tissue lysate Lane 5: Rat liver tissue lysate Lane 6: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722699) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human glioblastoma tissue with Rabbit anti-HES5 antibody (HA722699) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722699) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-HES5 antibody (HA722699) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722699) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.