CD134 Recombinant Rabbit Monoclonal Antibody [JE36-48]
cat.: HA722700
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE36-48
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 29 kDa
Isotype: IgG
Immunogen: Recombinant protein within human CD134 aa 1-250 / 277.
Positive control: HUT 102 cell lysate.
Subcellular location: Membrane.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P43489 Human
Alternative names: ACT 35 ACT35 ACT35 antigen ATC35 antigen CD 134 CD134 CD134 antigen IMD16 Lymphoid activation antigene ACT35 OX 40 OX40 OX40 antigen OX40 cell surface antigen OX40 homologue OX40L receptor TAX transcriptionally activated glycoprotein 1 receptor TAX transcriptionally-activated glycoprotein 1 receptor TNF receptor superfamily member 4 TNFRSF 4 TNFRSF4 TNR4_HUMAN Tumor necrosis factor receptor superfamily member 4 Txgp 1l Txgp1 Txgp1l
Images
HA722700_1.jpg Fig1: Western blot analysis of CD134 on different lysates with Rabbit anti-CD134 antibody (HA722700) at 1/1,000 dilution.

Lane 1: HUT 102 cell lysate
Lane 2: Jurkat cell lysate (negative)

Lysates/proteins at 20 µg/Lane.

Predicted band size: 29 kDa
Observed band size: 50 kDa

Exposure time: 14 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722700) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.