Complement factor B Recombinant Rabbit Monoclonal Antibody [JE54-96]
cat.: HA722704
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE54-96 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 86 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within |
| Positive control: | Human lung tissue lysate, Mouse lung tissue lysate, Rat lung tissue lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P00751 Human | P04186 Mouse Entrez Gene: 294257 Rat |
| Alternative names: | C3/C5 convertase Complement factor B Glycine-rich beta glycoprotein PBF2 Properdin factor B |
Images
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Fig1:
Western blot analysis of Complement factor B on different lysates with Rabbit anti-Complement factor B antibody (HA722704) at 1/1,000 dilution. Lane 1: Human lung tissue lysate Lane 2: Mouse lung tissue lysate Lane 3: Rat lung tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 86 kDa Observed band size: 86 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722704) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.